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KMID : 0613820110210121659
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Journal of Life Science
2011 Volume.21 No. 12 p.1659 ~ p.1665
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Development of a SCAR Marker Linked to Male Fertility Traits in 'Jinkyool' (Citrus sunki)
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Chae Chi-Won
Dutt Manjul
Yun Su-Hyun
Park Jae-Ho
Lee Dong-Hoon
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Abstract
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In Citrus, an F₁ segregation population of 150 plants was constructed from a cross between 'Kiyomi' (C. unshiu × C. sinensis) carrying the male sterility trait and 'Jinkyool' (C. sunki). Sequence-related amplification polymorphism (SRAP) combined with bulked segregant analysis was used to develop markers linked to male fertility. In the F₁ population, 66 out of 150 seedlings had aborted anthers and the ratio of male sterile plants to fertile plants in the progenies matched the expected Mendelian segregation ratio of 1:1 (x² =2.16 at p=0.05). From the profiling of the 197 SRAP primer sets, three SRAP primer sets (F4/R27, F39/R60, and F15/R37) that were closely linked to the target trait were identified and successfully converted into a sequence characterized amplified region (SCAR) marker for selection of male fertility in citrus. The SCAR marker, using the pMS 33U/pMS 1462L primer set specifically, produced a single 1.4-Kb fragment that was linked to male fertility. Our results suggested that this SCAR marker can be useful for marker-assisted selection of male sterile individuals in breeding F₁ progenies in Citrus.
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KEYWORD
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Bulked segregant analysis, Citrus unshiu, male sterility, 'Satsuma' mandarin
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